AICON-BIO™ Plant DNA Extraction Kit

Introduction

This kit uses a spin column that binds nucleic acids with high efficiency and a unique buffer system, which is

suitable for the extraction of genomic DNA from 50~100 mg of common plants. The kit is based on silica gel

column purification technology. No toxic phenol chloroform is needed in the extraction process. The entire

extraction process only takes 30~40 min, which can maximize the removal of impurities in plant tissues. The

extracted genomic DNA fragments are complete and high in purity, and can be directly For downstream PCR

amplification, qPCR, molecular markers and library construction experiments.

Highlights

1. Easy to operate: complete genomic DNA extraction from several samples within 30~40 min.
2. Safe and low toxicity: no toxic reagents such as phenol and chloroform are required.
3. High DNA purity: The extracted genomic DNA has no impurities, which is suitable for downstream experiments.

Q&A

Q1: Why is the column clogged?

A1:

1) Too much sample is used. It is recommended to extract according to the recommended amount of the manual.

2) The samples were rich in polysaccharides and polyphenols. Special kits are recommended for processing tissues rich in polysaccharides and polyphenols.

3) The centrifugation temperature is too low. The operation of this product is carried out at room temperature.

Q2: Why is the DNA extraction yield low?

A2:

1) The amount of sample is too small. It is recommended to extract in accordance with the recommended amount in the manual.

2) The quality of the sample material is not good. Choose fresh tissue samples as much as possible. After the samples are collected, they should be snap-frozen in liquid nitrogen and then stored at -80°C. It is recommended to extract as soon as possible to avoid repeated freezing and thawing.

3) Insufficient sample lysis. If the sample is excessive, reduce the amount of sample appropriately. After adding Buffer GP1, vortex for 1 min to fully mix it, and the lysis time can be appropriately extended.

Q3: Why is there RNA contamination in extracted DNA?

A3:

1) RNase A digestion was not added. Please add RNase A to digest according to the instructions.

2) The RNA content in the sample is too high. The amount of RNase A can be appropriately increased or the digestion time can be extended.

This kit is designed specifically for extracting genomic DNA from plant tissues with less secondary metabolites.