AICON-BIO™ Bacteria Genomic DNA Extraction Kit

Introduction

This kit can rapidly extract high-quality genomic DNA from various bacteria (gram-negative and gram-positive bacteria),

and can process 106~108 cells per time. The kit adopts an optimized buffer system, so that the DNA in the lysate can be

efficiently and specifically bound to the silica matrix adsorption column. The extraction process does not require the use of

toxic reagents such as phenol or chloroform, and the obtained DNA has high concentration and purity, and can be directly

used in down stream experiments such as enzyme digestion, PCR, library construction, SouthernBlot, and molecular labeling.

Highlights

1. High DNA quality: The extracted DNA has high concentration and purity, which is suitable for downstream

experiments that require high concentration, purity and integrity.

2. Safe and low toxicity: No toxic reagents such as phenol and chloroform are required.

Contents

Q&A

Q1: Why are the columns clogged?

A1:

1) Too much bacterial solution or insufficient sample lysis. It is recommended to extract according to the recommended

amount of the manual.

2) The centrifugation temperature is too low. The operation of this product is carried out at room temperature.

Q2:Why DNA yields are low?

A2:

1) The amount of bacterial liquid is too much or too little. It is recommended to extract according to the recommended

amount of the manual.

2) The bacterial liquid is not fresh. It is recommended to extract fresh or high-viability bacterial liquid (such as log-phase

bacteria).

Q3: Why Late Experiments Are Influenced by RNA?

A3:

1) RNase A digestion was not added. If the subsequent experiments need to remove the influence of RNA, RNase A can

be added to digest according to the instructions.

2) Too much RNA in the sample. The amount of RNase A can be appropriately increased or the digestion time can be

extended.

Q4: Why does the result show that there are more protein residues?

A4:

Too much bacterial fluid. It is recommended to reduce the amount of bacterial solution or repeat step 7 once.

This product is for research use only, not for clinical diagnosis.