AICON-BIO™ Animal Tissue/Cell Total RNA Extraction Kit （Double Column Type）

This product is a new rapid extraction kit for total RNA from animal tissues/cells.

It can remove genomic DNA contamination in one step through gDNA filter column without using β-mercaptoethanol.

Introduction

This kit can rapidly extract total RNA from ≤ 30 mg animal soft tissues (liver, spleen, kidney, brain, etc.) and

≤1×107 cultured cells. The kit is based on silica gel column purification technology, no need to use phenol

chloroform and β-mercaptoethanol in the extraction process, and the entire extraction process can be completed

within 25 minutes. Combined with DNA filtration technology, the kit can efficiently filter and remove genomic

DNA. The total RNA extracted is of high purity, free of protein and other impurities, and can be used for RT-PCR,

Northern Blot, Poly A purification, RNase protection analysis and in vitro translation, etc. experiment.

Contents

Storage Conditions

It can be stored for 18 months at room temperature (10~25℃).

Q&A

Q1: Why do the columns get clogged?

A1:

1) Too much sample. The lysate is viscous. Reduce the amount of sample or increase the amount of lysate. Too

much sample will reduce yield and purity.

2) Insufficient centrifugation of lysate. The tissue lysate needs to be centrifuged at high speed to remove

impurities, which will cause blockage of the column.

3) The centrifugation temperature is too low. The operation of this product is carried out at room temperature.

Q2: Why do gDNA filter cartridges clog during RNA extraction?

A2:

1) Increase the number of centrifugations, time or revolutions.

2) The filtrate can be sucked out and added to an appropriate amount of lysate to homogenize and then

transferred to a new gDNA filter column.