Aicon-Bio™ Restriction Endonucleases

Restriction endonucleases (Restriction enzymes) are a class of endonuclease that can recognize specific nucleotide sequences (4~8 base pairs) within double-stranded DNA and cleave DNA at specific sites. The key characteristic of restriction enzymes is their ability to accurately cut DNA and generate defined ends. The Aicon-Bio™ restriction endonucleases provided by BestEnzymes use only one universal reaction buffer, which provide a better experience for both single and double enzyme digestions. Moreover, Aicon-Bio™ restriction endonucleases are compatible with the buffers used in downstream experiments, achieving true one-tube convenience. The Aicon-Bio™ restriction enzymes can complete digestion with 5~15 minutes, which not only saves user’s time but also significantly reduces star activity.

Product Features

• Universal buffer makes multi-enzyme digestions more convenient. Additionally, it is compatible with downstream experiments, reducing the need for
  additional purification steps and saving time and effort.
• Fast digestion, completed within 5~15 minutes.
• The Aicon-Bio™ Color buffer allows for direct gel electrophoresis after digestion.

• Low star activity ensures prolonged reaction without generating non-specific cleavage.

Technical Features

Digestion can be completed accurately within 5~15 minutes                                               Consistent digestion results in Aicon-Bio™ Buffer and Aicon-Bio™ Color Buffer
 

Perform single, double and triple enzyme digestions using                                                Perform digestion using Aicon-Bio™ EcoRI, Aicon-Bio™ BamHI, and
Aicon-Bio™ EcoRI, Aicon-Bio™ Sall, and Aicon-Bio™ Sacl in                                               Aicon-Bio™ Xbal in Aicon-Bio™ Buffer and Aicon-Bio™ Color Buffer with
Aicon-Bio™ Buffer. Use 1 μg of plasmid as the substrate and carry out                           1 μg of plasmid substrate for 5 minutes. The same enzyme exhibits
the digestion for 5 and 15 minutes, respectively. Under all reaction                                  no significant difference in the digestion results between two buffers.
conditions, the substrate is completely cleaved accurately.

Ultra-low star activity

Using the fast restriction enzymes Hindll, Nhel, Pstl, Xbal, and Xhol from BestEnzymes, Company A, and
Company B respectively, perform a 16-hour digestion on λDNA substrate following the recommended protocols
by each brand. The results show that enzymes from BestEnzymes and Company A did not exhibit star activity.
The arrows present the bands generated by star activity.